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CAR-T細(xì)胞培養(yǎng)基

CAR-T(Chimeric Antigen Receptor T-Cell Immunotherapy)，即嵌合抗原受體T細(xì)胞mianyi療法，作為exingzhongliu的希望之星，CAR-T備受期待，高分文章頻出，行業(yè)利好消息不斷，但CAR-T研究方興未艾，需要用 的實(shí)驗(yàn)工具，減少實(shí)驗(yàn)摸索時間，提高CAR-T細(xì)胞培養(yǎng)效率，比如專業(yè)用于CAR-T細(xì)胞培養(yǎng)的LONZA X-VIVO 15 無血清免疫細(xì)胞培養(yǎng)基。

瑞士LONZA集團(tuán)生產(chǎn)的X-VIVO 15 化學(xué)成分限定培養(yǎng)基，由LONZA在美國和比利時的工廠生產(chǎn)，專為免疫細(xì)胞培養(yǎng)設(shè)計，屬于化學(xué)成分明確的無血清培養(yǎng)基，不含外源性生長因子、人造細(xì)胞增殖刺激因子、蛋白激酶C刺激劑等，近年來廣泛應(yīng)用于CAR-T細(xì)胞培養(yǎng)中，并在Cell、Nature等高分雜志中刊載（例如：Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia,Cell, Volume 173, Issue 6, 31 May 2018, Pages 1439–1453.e19；Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection,Nature 22 February 2017, 543, 113–117）。

LONZA X-VIVO 15 無血清免疫細(xì)胞培養(yǎng)基，由GMP級別工廠生產(chǎn)，已在美國FDA進(jìn)行備案，品質(zhì) ，受到國外市場的廣泛認(rèn)可，同時，在中國細(xì)胞 領(lǐng)域有著極高的知名度，越來越多的科研人員已開始使用X-VIVO 15 進(jìn)行CAR-T細(xì)胞mianyi療法相關(guān)研究。



LONZA X-VIVO 15 英文產(chǎn)品描述：
I. Introduction
Lonza’s R&D efforts and involvement with numerous clinical trials have provided us with the tools and expertise to support developments in adoptive immunotherapy, cancer therapy, genetic therapy and other cellular therapies. Our philosophy for the development of serum-free formulations for use in cellular therapy is to provide a nutritionally complete and balanced environment for the cells. Our X-VIVOTM Media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation or undefined supplements.

The products are devoid of any protein-kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical-grade human albumin, recombinant human insulin and pasteurized human transferrin. All X-VIVO. Medium products are manufactured under current GMPs and are listed with the FDA in a Product Master File. Permission to cross-reference the Master File may be obtained by contacting Lonza.


II. X-VIVO 15 Medium
X-VIVO 15 Medium is similar in composition to XVIVO 10 Medium and has been optimized for the proliferation of Tumor Infiltrating Lymphocytes (TIL) under serum-free conditions. The formulation supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors.

X-VIVO 15 Medium has also been used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes and natural killer(NK) cells. In addition, X-VIVO 15 Medium provides a serum-free environment for the expansion of HUT-78 and related human lymphocytic cell lines.


III. Additional Applications
A.Proliferation of PBL
B.Proliferation of TIL
C.Cryopreservation and transplantation of organs
D.Cultivation of human monocytes and macrophages
E.Cultivation of stem cells
F.Cultivation of dendritic cells


IV. Selected List of Cell Types Grown with X-VIVO Media
A.Human and murine LAK cells
B.Peripheral blood Lymphocytes
C.Human and murine TIL
D.Human T cells
E.Human and murine macrophages
F.Human and murine hematopoietic stem cells(colony formation and proliferation)
G.Cryopreservation of human tissue
H.Proliferation of draining


V. Storage
X-VIVO Medium should be stored at 2-8°C.
Protect from light.