作為一款高端創(chuàng)新型的產(chǎn)品,Cellartis DEF-CS培養(yǎng)系統(tǒng)使得單細胞干細胞操作成為常規(guī)的操作。該產(chǎn)品是專門針對人類誘導干細胞(hiPS)和人類胚胎干細胞(hES)研發(fā)的高效率增殖用培養(yǎng)基產(chǎn)品體系。該產(chǎn)品系統(tǒng)為all-in-one型,包括了所有組分,客戶不需要另外求購組分。該產(chǎn)品是成分確定的培養(yǎng)基產(chǎn)品,而且不需要飼養(yǎng)層。DEF-CS既可以實現(xiàn)單細胞培養(yǎng),也可以用于傳統(tǒng)的iPS培養(yǎng)模式以及大規(guī)模干細胞增殖。在使用DEF-CS培養(yǎng)基增殖干細胞時,幾乎沒有背景分化的問題,這使得細胞篩選不再必需。作為一款創(chuàng)新產(chǎn)品,利用DEF-CS培養(yǎng)干細胞時,可以使用酶消化法(enzymatic passaging)實現(xiàn)需要單細胞操作,這一特點十分有利于高通量細胞鑒別篩選(high-throughput screening)、轉(zhuǎn)染(transfection)、框架接種(scaffold seeding)等。
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■ 產(chǎn)品特點
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· Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation
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■ 產(chǎn)品應(yīng)用
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· Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold)
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