MesenCult™-ACF Chondrogenic Differentiation Medium is animal component-free (ACF) and specifically formulated for the in vitro differentiation of human mesenchymal stromal cells (MSCs; also known as mesencyhymal stem cells) into chondrogenic lineage cells, including chondrocytes. This medium is suitable for the differentiation of human bone marrow (BM)-, adipose tissue (AT)- and synovium (S)-derived MSCs previously culture-expanded in serum-containing medium (e.g. MesenCult™ Proliferation Kit [Catalog #05411]) or animal component-free MesenCult™-ACF Plus Medium [Catalog #05445]). MesenCult™-ACF Chondrogenic Differentiation Medium induces robust chondrogenic differentiation of human MSCs with as few as 3 x 10^5 cells and as early as day 14.
Advantages
? Animal component-free (ACF) formulation
? Robust chondrogenic differentiation with as few as 3 x 10^5 MSCs and as early as day 14
? Strong expression of chondrogenic transcripts - Acan, Col2a, Sox9 and Col10a; low expression of hypertrophic transcript Mmp13
? Completes optimized ACF workflow for MSC isolation, expansion, cryopreservation and chondrogenic differentiation
Components
MesenCult™-ACF Chondrogenic Differentiation Basal Medium, 95 mL
MesenCult™-ACF 20X Chondrogenic Differentiation Supplement, 5 mL